HCC1806-CAS9 cell line

EN CN
Scientist.com
Approved Supplier
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
ENCN
location： Home > Products > Cas9 Stable Cell Lines > HCC1806-CAS9
KO Cell Lines
Stable Cell Lines
Wild-type Cell Lines
Stem Cell Lines
Cas9 Stable Cell Lines
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • OVA Stable Cell Lines
  • Stable Cell Lines
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus
  • HCC1806-CAS9 cell line HCC1806-CAS9-产品图
HCC1806-CAS9 cell line HCC1806-CAS9-产品图
HCC1806-CAS9 Copy Product Info
Catalog#： YC-C073-Cas9-H
Size： 1*10^6
Culture method： 90%RPMI-1640+10% FBS
STR Report
Datasheet (Use Instruction)
  • HCC1806-CAS9 cell line HCC1806-CAS9-产品图
HCC1806-CAS9 Copy Product Info
Catalog#： YC-C073-Cas9-H
Size： 1*10^6
Culture method： 90%RPMI-1640+10% FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Endothelial cells, adherent Culture method 90%RPMI-1640+10% FBS
Passage ratio 1:2~1:4 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Endothelial cells, adherent
Culture method
90%RPMI-1640+10% FBS
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's HCC1806 cell line was constructed on the basis of wild-type HCC1806 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in HCC1806 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
Human Breast Cancer Cell Line Cas9 15.93004894 2.19846630
Human Breast Cancer Cell Line β-actin 13.73158264
HCC1806 Cas9 35.22600174 19.88505268
HCC1806 β-actin 15.34094906
Cutting Efficiency Validation
HCC1806-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the Human Breast Cancer Cell Line stable cell pool which is electroporated by NONO gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:HCC1806		 STR Info (Cell bank)
Cell Line:HCC 1806
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 11111111
D7S820 10121012
D16S539 10101010
VWA 16181618
TH01 8888
AMEL XXXX
TPOX 8889
CSF1PO 12121212
D12S391 1921
FGA 2525
D2S1338 1717
D21S11 2929
D18S51 1616
D8S1179 1415
D3S1358 1616
D6S1043 1212
PENTAE 1212
D19S433 1414
PENTAD 1515
D1S1656 15.315.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
Learn More>>>
Learn More>>>

Related Popular Products

Luciferase Stable Cell Lines

KO Cell Lines

  • ADPRS Knockout cell line (HCC1806)
    Request a Quote More details

    Organism: Human
    Gene Name: ADPRS
    Gene ID: 54936
    Size: 1*10^6
    Catalog#: YKO-H1057
    YKO-H1057(HCC1806)

Wild-type Cell Lines

EGFP Stable Cell Lines

Related Popular Products

Luciferase Stable Cell Lines

KO Cell Lines

  • ADPRS Knockout cell line (HCC1806)

    Organism: Human
    Gene Name: ADPRS
    Gene ID: 54936
    Size: 1*10^6
    Catalog#: YKO-H1057
    Request a Quote More details

Wild-type Cell Lines

EGFP Stable Cell Lines


WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now
Contact us
✖

Contact us

If we are unable to reach you via email, how else can we contact you?
✖
Submission Succeeded
Book A Virtual Meeting With Technical Support